現(xiàn)貨供應(yīng)人腦紅蛋白（NGB）ELISA試劑盒我公司主要經(jīng)營進(jìn)口原裝Elisa試劑盒全國：，種類齊全，價格實(shí)惠，購買我公司產(chǎn)品的新老客戶，可享免費(fèi)代測實(shí)驗(yàn)服務(wù)，可檢測人源，小鼠，大鼠，豬，狗，猴，兔，羊，雞，牛，鴨子，魚，植物等各種樣本。全程提供。如需其他Elisa試劑盒說明書及報價，可我公司

現(xiàn)貨供應(yīng)人腦紅蛋白（NGB）ELISA試劑盒操作步驟:
1.包被過程(注意設(shè)置空白對照,陰性對照):
將所用抗原用包被稀釋液稀釋到適當(dāng)濃度(一般所需抗原包被量為每孔20-200μg),每孔抗原加入100μl,置37℃,4h,或4℃,24h;棄去孔中液體.(為避免蒸發(fā),板上應(yīng)加蓋或?qū)迤椒旁诘撞坑袧窦啿嫉慕饘贊窈兄?
2.封閉酶標(biāo)反應(yīng)孔:
5%小牛血清置37℃封閉40min.封閉時將封閉液加滿各反應(yīng)孔,并去除各孔中的氣泡,封閉結(jié)束后用洗滌液滿孔洗滌3遍,每遍3min.
洗滌方法:吸干孔內(nèi)反應(yīng)液,將洗滌液注滿板孔,放置2min略作搖動,吸干孔內(nèi)液,傾去液體后在吸水紙上拍干
洗滌次數(shù)3次
3.加入待檢測樣品(建立合適的濃度梯度):
檢測時一般采用1:50-1:400的稀釋度,應(yīng)采用較大稀釋體積進(jìn)行,一般保證樣品吸取量>20μl.
將稀釋好的樣品加入酶標(biāo)反應(yīng)孔中,每樣品少加雙孔,每孔100μl,置于37℃,40-60min.用洗滌液滿孔洗滌3遍,每遍3min.
4.加入酶標(biāo)抗體:
酶標(biāo)抗體:根據(jù)酶結(jié)合物提供商提供的參考工作稀釋度進(jìn)行.37℃,30-60min之間.短于30min往往結(jié)果不穩(wěn)定.每孔加100μl.洗滌同前.
5.加入底物液(現(xiàn)用現(xiàn)配):
*TMB-過氧化氫尿素溶液,OPD-過氧化氫底物液系統(tǒng)次之.
底物加入量:每孔100μl,置37℃避光放置3-5分鐘,加入終止液顯色.
6.終止反應(yīng):
每孔加入終止液50μl終止反應(yīng),于20min內(nèi)測定實(shí)驗(yàn)結(jié)果.
7.結(jié)果判斷:
OPD顯色后采用492nm波長,TMB反應(yīng)產(chǎn)物檢測需要450nm波長.檢測時一定要首先進(jìn)行空白孔系統(tǒng)調(diào)零.用測定標(biāo)本孔的吸收值與一組陰性標(biāo)本測定孔平均值的比值(P/N)表示,當(dāng)P/N大于2時作為抗體的效價.(數(shù)值的大小依具體檢測要求而定.)Rat cross-linked C-opeptides of Type Ⅰ collagen,CⅠCP ELISA試劑盒
Rat deoxypyridinoline,DPD ELISA試劑盒 
Rat pyridinium crosslink/PyriLinks,PY ELISA試劑盒
Rat Osteopontin,OPN ELISA試劑盒
Rat Bone-specific Alkphase B,ALP-B ELISA試劑盒
Rat transferrin receptor,TFR ELISA試劑盒
Rat Cystatin C,Cys-C ELISA試劑盒
Rat Factor Ⅷ-related Antigen,FⅧAg ELISA試劑盒
Rat Pyruvate Kinase,M2-PK ELISA試劑盒
Rat acetylcholine receptor antibody,AChRab ELISA試劑盒
Rat Gamma-aminobutyric acid,GABA ELISA試劑盒
Rat 5-Hydroxytryptamine,5HT ELISA試劑盒
Rat substance P receptor,SP-R ELISA試劑盒
Rat Substance P,SP ELISA試劑盒
Rat alpha-L-fucosidase,AFU ELISA試劑盒
Rat Thyroid-Peroxidase,TPO ELISA試劑盒
Rat Proinsulin,PI ELISA試劑盒
Rat anti-IgE receptor antibody ELISA試劑盒
Rat anti-IVIgG antibody ELISA試劑盒
Rat p53/tumor protein,p53/TP53 ELISA試劑盒
Rat Cyclic guanosine monophosphate,cGMP ELISA試劑盒
Rat nitric oxide synthase,NOS ELISA試劑盒
Rat inducible nitric oxide synthase,iNOS ELISA試劑盒
Rat Endothelial nitric oxide synthase,eNOS ELISA試劑盒
Rat lipoprotein-associated phospholipase A2,Lp-PL-A2 ELISA試劑盒
Rat heat shock protein 90,hSP-90 ELISA試劑盒
Rat heat shock protein 70,hSP-70 ELISA試劑盒
Rat heat shock protein 40,hSP-40 ELISA試劑盒
Rat heat shock protein 20,hSP-20 ELISA試劑盒
Rat amyloid beta peptide 1-40,Aβ1-40 ELISA試劑盒
Rat Cyclin-D3 ELISA試劑盒
Rat Cyclin-D2 ELISA試劑盒
Rat Cyclin-D1 ELISA試劑盒
Rat myelin basic protein,MBP ELISA試劑盒
Rat Alpha-fetoprotein,AFP ELISA試劑盒
Rat Prostatic Acid Phosphatase,PAP ELISA試劑盒
Rat Free Prostate Specific Antigen,fPSA ELISA試劑盒
Rat prostate specific antigen,PSA ELISA試劑盒
Rat ovarian cancer marker-CA125 ELISA試劑盒
Rat mammary carcinoma Marker-CA153 ELISA試劑盒
Rat Gastrointestinalcancer Marker-CA199 ELISA試劑盒
Rat heme oxygenase 1,HO-1 ELISA試劑盒
Rat Phospholipidase A2,PL-A2 ELISA試劑盒
Rat Tissue factor pathway inhibitor,TFPI ELISA試劑盒
Rat Aquaporin 5,AQP-5 ELISA試劑盒
Rat Aquaporin 4,AQP-4 ELISA試劑盒
Rat Aquaporin 3,AQP-3 ELISA試劑盒