抗巨噬細胞抗體（anti-macrophageAb）ELISA檢測試劑盒齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體,分子學生物試劑盒,金標法檢測試劑盒,比色法檢測試劑盒,細胞菌株,培養(yǎng)基,細胞因子,生化試劑,耗材等生物試劑產(chǎn)品.誠信經(jīng)營，價格實惠，服務周到，質(zhì)量保障！提供各種實驗代測服務.歡迎新老客戶！齊一生物科技（上海）有限公司，專業(yè)生產(chǎn)研發(fā)生化科研試劑全國

抗巨噬細胞抗體（anti-macrophageAb）ELISA檢測試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫存：現(xiàn)貨
產(chǎn)品價格：詢價
使用范圍：僅供科研檢測,不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專門快遞免費送貨上門
產(chǎn)品價格：*,洽談！
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取.全國：代理多種品牌進口原裝、分裝“ELISA試劑盒”.齊一生物科技有限公司作為酶聯(lián)免疫供應商,憑借*的技術,*的售前,售中,售后免費代測服務,共同鑄就高品質(zhì)的產(chǎn)品.多年專業(yè)酶免服務,質(zhì)量保證,可免費提供代測服務,一周內(nèi)出結果.歡迎訂購.
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標準品的數(shù)量決定所需的板條數(shù).每個標準品和空白孔建議做復孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復孔的盡量做復孔.標本用標本稀釋液1：1稀釋后加入50ul于反應孔內(nèi).
加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內(nèi).立即加入50ul的生物素標記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結果.
在450nm波長處測定各孔的OD值.
ELISA操作步驟
酶聯(lián)免疫吸附實驗材料,試劑,溶液
1．酶標板,100μltip頭,1mlEp管,濕盒
2．包被稀釋液（0.05mol/L碳酸鈉-碳酸氫鈉buffer,pH9.6）
碳酸鈉0.15g,碳酸氫鈉0.29g,疊氮鈉0.02g,加雙蒸水100ml,調(diào)pH9.6
3.封閉液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗滌液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
5.樣本稀釋液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
6.酶標第2抗體(羊抗兔)稀釋范圍1:5,000-1:100,000
7.底物液(TMB-過氧化氫尿素溶液)
①底物液A:TMB20mg,無水乙醇10ml,加雙蒸水100ml.
②底物液B(0.1mol/L檸檬酸-0.2mol/L磷酸二氫鈉緩沖液,pH5.0-5.4)
Na2HPO41.46g,檸檬酸0.933g,0.75%過氧化氫尿素0.64ml,加三蒸水100ml,調(diào)pH5.0-5.4
③底物A和B按1:1混合即成TMB-過氧化氫尿素溶液.
8.終止液(2mol/LH2SO4溶液)雙蒸水200ml,濃硫酸34ml,(緩慢滴加并不斷攪拌),加雙蒸水300ml
9.0.9%生理鹽水

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anti-PALS/MPP5
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anti-PAR6alpha/PARD6A
anti-CLIM1/PDLIM1
anti-4E-T/EIF4ENIF1
anti-KPNA1/Importin alpha 5
anti-KPNA2/IPOA1
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anti-SIRT4
anti-PIK3C2A
anti-NOXO1
anti-Dispatched homolog 1
anti-SSX2IP
anti-FHL2
anti-DDX5/p68 RNA helicase
anti-AKAP3/SOB1
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anti-TIP30/CC3
anti-VDAC2
anti-AKAP8/AKAP95
anti-PITPNM/PITPNM1
anti-MUTYH
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anti-NIR1/PITPNM3
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anti-ARP1 homolog A
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anti-Swiprosin 1/EFHD2
anti-TOM1L2
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anti-MBL2/Mannan-Binding Lec  tin
anti-Synaptogyrin 2/Cellugyr  in
anti-Synaptogyrin 4
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anti-pan ADH
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anti-OIP106/TRAK1
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anti-HPS6
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anti-AP-2 gamma
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