D-蟲熒光素游離酸CAS號：2591-17-5英文名稱：D-Luciferin；Firefly Luciferin；(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid；4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid
其他名稱：(S)-4,5-二氫-2-(6-羥基苯并噻唑-2-基)噻唑-4-甲酸；D-熒光素；D-冷光素
CAS號：2591-17-5
C11H8N2O3S2=280.32
級別：BR
純度：≥98.0%
熔點：201～204°C
Maximum UV absorption：533 nm
性狀(以下信息僅供參考)：類白色淡黃色粉末。溶于甲醇：10mg/ml；也溶于DMSO：50mg/ml
用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)化學發光試劑
保存：2～8℃。保質期2年
990512    Filter-Tips, 50 μl (960)
337620    qBiomarker SYBR Green Fluor Mastermix(2)
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D-蟲熒光素游離酸CAS號：2591-17-5939016    Buffer ATL ( 4 x 50 ml),GPR
4500163    artus HSV-1/2 TM PCR Kit (24) CE
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9243653    Comprehen Valid., Other Extraction Sys    """The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to plete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and plete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan enpasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via ) any validation data requested after the on-site visit. A QIAGEN specialist will plete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring pliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (/CD/flashdrive) to the laboratory. The laboratory is responsible for final pilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating pliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following pletion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """
386516    Investigator STR GO! Lysis Buffer (200)
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280377    virotype BVDV RT-PCR Kit (480)
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9011759    Reagent holder, 4-tube 13ml    Holder for acmodating 4 microcentrifuge tubes (13 ml) on the worktable; must be used with the reagent holder tray
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132605    NeXtal Stock Sodium ac. H2O (50)    1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)
800141    Investigation grade sequencing, w/ prep    "Single-stranded sequencing of an unknown sequence
QIAGEN DNA template preparation
Design and synthesis of walking primers
Data assembly and sequence alignment
Accuracy >99%"
767134    PAXgene Tissue DNA Kit (50)
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330513    RT2 SYBR Green Fluor qPCR Mastermix (24)
330502    RT2 SYBR Green qPCR Mastermix (12)
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