Porcine IL-12Rβ2 gene was cloned from mRNA preparation of mitogen-activated peripheral blood mononuclear cells (PBMCs), and its complete nucleotide sequence was determined. To confirm the biological function, the entire open reading frame (ORF) was re-cloned into a mammalian expression vector, pcDNA3.1/Zeo(+), at the downstream of CMV promoter, and introduced to a Th1-like human lymphoma cell line, Jurkat E6-1. Antibiotic-resistant cells retaining the expression construct were selected then, isolated by the limiting dilution method. An established clone (10B10) constitutively expressed chimeric IL-12Rs composed of intrinsic (human) β1 and extrinsic (porcine) β2 subunits, and produced interferon (IFN)-γ in response to IL-12 of both species with optimal PHA/PMA stimulation. The production of IFN-γ was observed as early as 42 h after culture and appeared to be dose-dependent within the range between 20 and 2000 pg/ml. Thus, this clone not only reacts with IL-12 of both species but also provides a useful tool for quick and sensitive detection of IL-12 bioactivity.Porcine IL-12Rβ2 gene was cloned from mRNA preparation of mitogen-activated peripheral blood mononuclear cells (PBMCs), and its complete nucleotide sequence was determined. To confirm the biological function, the entire open reading frame (ORF) was re-cloned into a mammalian expression vector, pcDNA3.1/Zeo(+), at the downstream of CMV promoter, and introduced to a Th1-like human lymphoma cell line, Jurkat E6-1. Antibiotic-resistant cells retaining the expression construct were selected then, isolated by the limiting dilution method. An established clone (10B10) constitutively expressed chimeric IL-12Rs composed of intrinsic (human) β1 and extrinsic (porcine) β2 subunits, and produced interferon (IFN)-γ in response to IL-12 of both species with optimal PHA/PMA stimulation. The production of IFN-γ was observed as early as 42 h after culture and appeared to be dose-dependent within the range between 20 and 2000 pg/ml. Thus, this clone not only reacts with IL-12 of both species but also provides a useful tool for quick and sensitive detection of IL-12 bioactivity.
廈門侖昌碩生物科技有限公司集研發、生產、銷售、服務及品牌代理為一體的生物技術公司，目前可提供各種抗體、免疫學相關的檢測試劑盒、胎牛血清，動物抗血清等高品質產品及服務，涵蓋分子生物學、細胞生物學、免疫學等生命科學領域。
公司非常注重企業信息化的建設及企業平臺的建設，我們內部采用了*的信息處理平臺，保證客戶的需求可以準確、高效處理。
公司成立之初，立足外貿，和部分歐美及亞非國家的客戶建立了良好的合作，隨著國內生物領域的蓬勃發展，逐步把重心轉移到國內來，為每一位科研人員獻上高品質的產品及服務是我們的使命。
集研發、生產、銷售、服務及品牌代理為一體的生物技術公司，目前可提供各種抗體、免疫學相關的檢測試劑盒、胎牛血清，動物抗血清等高品質產品及服務，涵蓋分子生物學、細胞生物學、免疫學等生命科學領域。
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