3-Methyladenine (3-甲基腺嘌呤）
注：本產品僅用于科研，不可用于臨床
生物活性
3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K
IC50 & Target[1]
PtdIns3Kγ 60 μM (IC50, Cell Assay) | Vps34 25 μM (IC50, Cell Assay) | Autophagy
| Mitophagy
| Human Endogenous Metabolite
|
體外研究
(In Vitro)
3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
3-Methyladenine induces cell death is independent of autophagy inhibition[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].
Note:
在體外細胞培養實驗中，3-MA 常用的濃度為 0.5-10 mM，所以優先推薦直接稱取本次實驗所需粉末量，用培養基全都溶解，0.22 μm 過濾器過濾除菌之后使用。建議溶液現配現用，盡快用完。
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[2]
| Cell Line: | HeLa cells |
| Concentration: | 0 mM, 2.5 mM or 5 mM, 10 mM |
| Incubation Time: | 0 hour, 24 hours and 48 hours |
| Result: | Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation. |
體內研究
(In Vivo)
3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)[3] |
| Dosage: | 1.5?mg/100?g (1000 μM) |
| Administration: | Intraperitoneal injection |
| Result: | Alleviated Sodium Taurocholate-Induced SAP. |
分子量
149.15
性狀
Solid
Formula
C6H7N5
CAS 號
5142-23-4
中文名稱
3-甲基腺嘌呤
運輸條件
Room temperature in continental US; may vary elsewhere.
儲存方式
| Powder | -20°C | 3 years |
|---|---|---|
| 4°C | 2 years | |
| In solvent | -80°C | 6 months |
| -20°C | 1 month |
溶解性數據
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