磷酸酶抑制劑 Cocktail I
注：本產品僅用于科研，不可用于臨床
磷酸酶抑制劑 Cocktail I簡介
磷酸酶抑制劑Cocktail Ⅰ（100X，DMSO儲存液）可以有效的抑制堿性磷酸酶和serine/threonine磷酸酶，如：PP1、 PP2A，用于維持蛋白的磷酸化狀態。
| Description & Advantages | Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM). Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO. | |||||||||||||||
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| Storage | The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months. | |||||||||||||||
| Protocol | Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition. Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc. | |||||||||||||||
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磷酸酶抑制劑 Cocktail I
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