蛋白名稱:EGFR人重組蛋白，10001-H02H
蛋白構(gòu)建:A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was fused with the Fc region of human IgG1 at the C-terminus.
表達(dá)宿主:HEK293 Cells
蛋白純度:> 97 % as determined by SDS-PAGE. >85% as determined by SEC-HPLC
蛋白活性:1.Immobilized Recombinant Human TGFA / TGF-alpha Protein (Cat: 11252-HNAE) at 2 μg/ml (100 μl/well) can bind Recombinant Human EGFR / HER1 / ErbB1 Protein (Fc Tag) (Cat: 10001-H02H), the EC50 is 2-8 ng/mL. 2.Immobilized Recombinant Human EGFR / HER1 / ErbB1 Protein (Fc Tag) (Cat: 10001-H02H) at 1 μg/mL (100 μL/well) can bind Cetuximab derived Anti-EGFR Antibody, Human IgG1, Biotinylated, the EC50 is 12-36 ng/mL(Routinely tested). 3.Immobilized Recombinant Human EGFR / HER1 / ErbB1 Protein (Fc Tag) (Cat: 10001-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human EGF/Epidermal Growth Factor Protein (Fc Tag),Biotinylated (Cat: 10605-H01H-B), the EC50 is 150-450 ng/mL(Routinely tested).
蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method
預(yù)測N端:Leu 25
蛋白分子量:The recombinant human EGFR/Fc chimera is a homodimeric protein. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110-140 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP號(hào):NP_005219
蛋白氨基酸序列:
Met1-Ser645
蛋白標(biāo)簽:C-human IgG1-Fc

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