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產品詳情相比近年來有長足進展的生物樣品純化和晶體X射線衍射技術，生物大分子晶體的培養依然是一個試驗探索的過程，極大程度地依賴于經驗并帶有較大的盲目性

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產品詳情

相比近年來有長足進展的生物樣品純化和晶體X射線衍射技術，生物大分子晶體的培養依然是一個試驗探索的過程，極大程度地依賴于經驗并帶有較大的盲目性。蛋白結晶所需的各種成分，如沉淀劑、鹽、緩沖液體系、有機溶劑以及各種添加劑，構成一個龐大的多維集合。雖然現在市場上有一些商業化的晶體生長條件篩選盒,但它們只能構成一個一個獨立的小子集, 遠遠無法覆蓋非常復雜、巨大的蛋白結晶條件集合。

博亞的BioXtal™結晶條件篩選盒是為蛋白晶體生長條件篩選而特別開發的一個晶體生長條件組合。其成分組成是以大量發表和未發表的結晶統計數據為基礎發展而成。它既包含離散矩陣篩選模式也包括系統性篩選模式，既含*效的結晶條件也含許多其它結晶條件篩選盒所未有的條件。擴展了篩選范圍從而增大了發現結晶條件的幾率。

該試劑盒有96個條件以適合96孔板自動化篩選設備，pH范圍涵蓋蛋白晶體最易生長范圍4.5-8.5。所使用的化學試劑來自國標*化學試劑公司（Fluka、Sigma-Aldrich等），并且每種溶液都經過0.22μ慮膜過慮滅菌處理。96個條件分別密封在螺旋蓋試管中，每管不少于10毫升，可隨便使用。

在最近進行的大量蛋白晶體篩選測試研究中，博亞BioXtal™結晶條件篩選盒曾結晶出了超過50%的可結晶蛋白。被認為是高效結晶條件組合，是一個對現有商業化的結晶條件篩選盒的有力補充和發展。

BioXtal^TM結晶條件篩選試劑盒

1. 0.9M (NH₄)₂SO₄ ,0.1M Na-acetate pH4.5

2. 0.9M (NH₄)₂SO₄ ,0.02M HEPES pH7.5 ,0.7M NaNO₃

3. 1.5M (NH₄)₂SO₄ ,0.1M MES pH6.5

4. 1.5M (NH₄)₂SO₄ ,0.1M Bis-tris pH7.0,

0.7%(w/v) Octyl-β-D-glucopyranoside

5. 1.5M (NH₄)₂SO₄ ,0.1M Tris-HCl pH7.5,

0.05M MgCl₂

6. 2.0M (NH₄)₂SO₄ ,0.1M Na-citrate pH5.0,

0.5M NaNO₃

7. 2.0M (NH₄)₂SO₄ ,0.1M Bis-tris pH5.5

8. 2.0M (NH₄)₂SO_{4 ,}0.1M Bis-tris pH6.5

9. 2.0M (NH₄)₂SO₄ ,0.02M HEPES pH7.5

10. 2.0M (NH₄)₂SO₄ ,0.1M Tris-HCl pH8.0,

0.02M CaCl₂ ,0.5% Octyl-β-D-glucopyranoside

11. 2.0M (NH₄)₂SO₄ ,0.1M Tris-HCl pH8.5

12. 2.0M (NH₄)₂SO₄

13. 2.88M (NH₄)₂SO₄ ,0.1M Na-citrate pH5.0

14. 2.88M (NH₄)₂SO₄ ,0.02M HEPES pH7.5

15. 15.0%(v/v) Jeffamine M600 ,0.1M HEPES pH7.0

16. 30.0%(w/v) Jeffamine ED-2001 ,0.1M HEPES pH7.0

17. 1.8M Li₂SO₄ ,0.05M Na Cacodylate pH6.0,

0.01M MgSO₄

18. 2.0M Li₂SO₄ ,0.05M MES pH5.6 ,0.01M MgCl2

19. 15.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH7.5 ,

0.2M KCl

20. 20.0%(w/v) PEG 2000 MME ,0.1M Tris-HCl pH8.5,

0.2M trimethylamine n-oxide

21. 28.0%(w/v) PEG 2000 MME ,0.1M Bis-tris pH6.5

22. 30.0%(w/v) PEG 2000 MME ,

0.05M Na Cacodylate pH6.0 ,0.05M MgCl₂

23. 30.0%(w/v) PEG 2000 MME ,0.15M KBr

24. 20.0%(w/v) PEG 5000 MME ,0.1M Bis-tris pH6.5

25. 20.0%(w/v) PEG 5000 MME ,0.1M HEPES pH7.0,

0.5M NaNO₃

26. 25.0%(w/v) PEG 5000 MME ,0.1M Tris-HCl pH7.5

27. 25.0%(v/v) PEG 550 MME ,0.1M Na-citrate pH5.0

28. 25.0%(v/v) PEG 550 MME ,0.1M Tris-HCl pH8.5

29. 30.0%(v/v) PEG 550 MME ,0.1M Bis-tris pH7.0 ,

0.05M MgCl₂

30. 10.0%(v/v) MPD ,0.1M Na-citrate pH5.0

31. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 0.1M CaCl₂

32. 20.0%(v/v) MPD ,0.05M Na Cacodylate pH6.0

33. 25.0%(v/v) MPD ,0.1M Na-citrate pH5.0

34. 25.0%(v/v) MPD ,0.1M MES pH6.5 ,0.75M NaNO₃

35. 25.0%(v/v) MPD ,0.02M HEPES pH7.5 ,0.05M MgCl₂

36. 30.0%(v/v) MPD ,0.1M Na-acetate pH4.5

37. 30.0%(v/v) MPD ,0.1M MES 6.5 ,0.02M CaCl₂

38. 30.0%(v/v) MPD ,0.1M Tris-HCl pH8.5 ,0.02M MgCl₂

39. 40.0%(v/v) MPD ,0.01M CaCl₂

40. 10.0%(v/v) MPD ,0.1M Tris-HCl pH7.5 ,0.01M MgCl₂ ,

0.05M NH₄-acetate

41. 1.2M NaCl ,0.1M MES pH6.5

42. 1.2M NaCl ,0.6M NaNO₃ ,

0.25%(w/v) Octyl-β-D-glucopyranoside

43. 1.5M NaCl ,0.1M Na-acetate pH4.5

44. 1.5M NaCl ,0.1M Bis-tris pH7.0

45. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.01M CaCl₂ ,

0.4%(w/v) Octyl-β-D-glucopyranoside

46. 1.5M NaCl ,0.1M Tris-HCl pH8.5 ,0.6M NaNO₃

47. 1.5M NaCl 0.1M NH₄-acetate ,

0.4% Octyl-β-D-glucopyranoside

48. 2.0M NaCl ,0.02M HEPES 7.5

49. 2.0M NaCl ,0.3M NaNO₃

50. 2.5M NaCl ,0.1M Na-citrate pH5.0 ,

0.9M NaNO₃

51. 2.5M NaCl ,0.1M Bis-tris pH6.5 ,0.3M NaNO₃

52. 2.5M NaCl ,0.1M Bis-tris pH7.0 ,

0.1M NH₄-acetate

53. 2.5M NaCl ,0.02M HEPES pH7.5 ,0.05M MgCl₂

54. 2.5M NaCl ,0.02M HEPES pH7.5

55. 2.9M NaCl ,0.1M Tris-HCl pH8.5,

0.4%(w/v) Octyl-β-D-glucopyranoside

56. 3.0M NaCl ,0.1M Na-citrate pH5.0 ,0.5M NaNO₃

57. 3.1M NaCl ,0.1M Bis-tris pH7.0

58. 3.5M NaCl ,0.1M Bis-tris pH5.5

59. 3.5M NaCl ,0.1M Tris-HCl pH7.5 ,0.05M MgCl₂

60. 30.0%(w/v) PEG 1000 ,0.1M Tris-HCl pH7.5 ,

5.0%(v/v) Glycerol

61. 17.0%(w/v) PEG 10000 ,0.1M Bis-tris pH5.5 ,

0.1M NH₄-acetate

62. 15.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.85M NaNO₃

63. 17.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

64. 20.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5 ,

0.05M MgCl₂

65. 20.0%(w/v) PEG 3350 ,0.2M Na-formate

66. 21.0%(w/v) PEG 3350 ,0.02M HEPES pH7.5,

0.7% Octyl-β-D-glucopyranoside

67. 22.0%(w/v) PEG 3350 ,0.1M MES pH6.5 ,0.08M CaCl₂ ,

0.7% Octyl-β-D-glucopyranoside

68. 24.0%(w/v) PEG 3350 ,0.01M CaCl₂

69. 25.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

70. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

0.2M Li₂SO₄

71. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

0.2M NaCl

72. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5

73. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5,

0.2M NH₄-acetate

74. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH5.5 ,

0.2M MgCl₂

75. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

0.2M MgCl₂

76. 25.0%(w/v) PEG 3350 ,0.1M Bis-tris pH6.5 ,

0.2M NH₄-acetate

77. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

0.2M MgCl₂

78. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,0.2M NaCl

79. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5

80. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5 ,

0.2M NH₄-acetate

81. 25.0%(w/v) PEG 3350 ,0.1M HEPES pH7.5,

0.2M (NH₄)₂SO₄

82. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

0.2M NaCl

83. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5

84. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

0.2M NH₄-acetate

85. 25.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH8.5 ,

0.2M MgCl₂

86. 28.0%(w/v) PEG 3350 ,0.1M Bis-tris pH 7.0 ,

0.02M CaCl₂

87. 30.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

88. 30.0%(w/v) PEG 3350 ,0.4M NaNO₃

89. 40.0%(w/v) PEG 3350 ,0.1M Na-citrate pH5.0

90. 40.0%(w/v) PEG 3350 ,0.1M Tris-HCl pH7.5 ,

0.4M NaNO₃

91. 30.0%(v/v) PEG 400 ,0.05M Na Cacodylate pH6.0

92. 30.0%(v/v) PEG 400 ,0.1M HEPES pH7.5 ,0.2M MgCl₂

93. 35.0%(v/v) PEG 400 ,0.1M Bis-tris pH6.5 ,

0.1M NH₄-acetate

94. 25.0%(w/v) PEG 4000 ,0.1M Na-acetate pH4.6 ,

0.2M (NH₄)₂SO₄

95. 30.0%(v/v) pentaerythritol ethoxylate (15/4 EO/OH) ,

0.05M Bis-tris pH6.5 ,0.05M (NH₄)₂SO₄

96. 35.0%(v/v) pentaerythritol propoxylate (5/4 PO/OH) ,

0.05M HEPES pH7.5 ,0.2M KCl

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BioXtal™(高通量)蛋白結晶條件篩選試劑盒

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