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TAMRA-Azide-Desthiobiotin

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (K_d = 10¹¹ vs. 10¹⁵M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Molecular weight	1145.33
Chemical composition	C57H82N11O14
CAS	N/A
Solubility	DMSO, DMF, DCM, THF, Chloroform
Purity	>95%
Appearance	Red amorphous solid to red oil
Storage conditions	-20C
Shipping conditions	Ambient temperature

Desthiobiotin是一種單環、無硫生物素的類似物，它與鏈霉菌素結合的特異性幾乎相同，但親和力卻不如生物素(Kd分別為1011和1015M)。因此，在與自由生物素競爭驅替的溫和條件下，可以很容易地、特別地從鏈霉毒素親和樹脂中分離出脫硫生物素。在生物樣品的下拉實驗中，desthiobiotin的軟釋放特性也有助于減少內源性生物素化分子的共提純，這些分子在游離生物素的目標蛋白復合物的洗脫作用下仍與鏈球菌肽結合。

分子量	1145.33
分子式	C57H82N11O14
CAS	N/A
溶解度	DMSO, DMF, DCM, THF, Chloroform
含量	>95%
外觀	紅色無定形固體或紅油色有油質
儲存	-20C
運輸	環境溫度

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Click Chemistry Tool

DBCO Reagents

Amine Reactive

DBCO-C6-NHS Ester

• DBCO-NHS Ester
• DBCO-Sulfo-NHS Ester
• DBCO-PEG4-NHS Ester
• DBCO-PEG5-NHS Ester
• DBCO-PEG13-NHS Ester
• PC DBCO-NHS Ester
• DBCO-C6-Acid
• DBCO-Acid
• DBCO-PEG5-Acid

Carboxyl/Carbonyl Reactive

• DBCO-Amine
• DBCO-PEG4-Amine
• Sulfo DBCO-PEG4-Amine
• Sulfo DBCO-Amine

 Sulfhydryl Reactive

• Sulfo DBCO-Maleimide

DBCO-Maleimide

• Sulfo DBCO-PEG4-Maleimide
• DBCO-PEG4-Maleimide
• Bis-sulfone-PEG4-DBCO
• ThioLinker-DBCO

Fluorescent Probes

• MB 532 DBCO

MB 488 DBCO

• MB 543 DBCO
• MB 594 DBCO
• Carboxyrhodamine 110 DBCO
• TAMRA DBCO
• Cy3 DBCO
• Cy5 DBCO
• Cy5.5 DBCO
• Cy7 DBCO

 Biotinylation Reagents

• DBCO-PEG4-Biotin

WS DBCO-Biotin

• DBCO-PEG4-Desthiobiotin
• Dde Biotin-DBCO
• PC DBCO-Biotin
• WS PC DBCO-Biotin
• Diazo Biotin-DBCO

PEGylation Reagents

• DBCO-mPEG, 5 kDa

DBCO-mPEG, 10 kDa

• DBCO-mPEG, 20 kDa
• DBCO-mPEG, 30 kDa

 Bifunctional Crosslinkers

• Methyltetrazine-DBCO

Methyltetrazine-PEG12-DBCO

• DBCO-PEG4-DBCO
• TCO-PEG12-DBCO

 Miscellaneous Reagents

• DBCO-PEG4-Hydroxyl